Simplified global gene expression profiling
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چکیده
• Simple workflow and analysis—the simplicity and speed of PCR enables a rapid method for gene expression profiling with a ≤2-day workflow from sample to analysis. Intuitive bioinformatics through point-andclick run setup and automated secondary data analysis in Torrent SuiteTM Software with an associated plug-in, the AmpliSeqRNA, enables unambiguous gene expression analysis and visualization from sequence-read data (Figures 7–9, Supplementary information). • Robust performance with limited or degraded samples—the power of Ion AmpliSeqTM technology enables reproducible detection of highand lowabundance transcripts from difficult samples such as formalin-fixed, paraffin-embedded (FFPE) tissue sections or from samples with limited input—as little as 10 ng total RNA.
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